- make up a litre of MacConkey Broth (Enzymatic Digest of Gelatin 20g; Lactose 10 g; Oxbile 5 g; Bromocresol Purple 0.01 g) which because of the bromocresol starts off an agreeable royal purple in colour. Only a few organisms will grow happily on lactose (milk sugar), and the Ox bile inhibits the half of the bacterial world, some of which can handle lactose, that are blue after Gram-staining. The digest of gelatin (ox hooves, possibly from the same animals that contributed their gall-bladders for the bile) provides all the other nutrients - a bit of protein, some vitamins. There's a century of science behind this: originally the indicator was litmus, which was replaced by 'neutral' red, which in turn was found to inhibit a bunch of the bacteria that poor MacConkey was trying to grow.
- dole it out into 10ml test-tubes.
- sink an inverted Durham tube the liquid in each tube.
- sterilize them all.
- add your possibly contaminated sample (we use sheep shit and other likely sources)
- Incubate at 40oC for a day or two.
Eijkman was born in Nijkerk on the shores of the Zuider Zee in the Netherlands. He trained as a medical officer for the army and shipped out to the Dutch East Indies in 1883 at the age of 25. He caught a dose of malaria and within two years shipped back home sick. It was good timing because he was able to go back to college and studied for a time under the giant of the microbiological world, Robert "Postulates" Koch in Berlin. While there, he met Professors Pekelharing and Winkler who had been tasked to work out the causes of beri-beri: a devastating disease of the tropical East Indies which was a huge drain on the Dutch colonial economy. Eijkman joined their team and in short order found himself on a boat back to Batavia. When the Big Guns went back to Europe, Eijkman stayed on as the newly appointed director of the Medical Laboratory; having resigned from the army, he could devote his energies to scientific research.
I think he was a great scientist who looked to the evidence for his understanding and wasn't swayed by what Everybody Knew To Be True. He carried out some careful metabolic and physiological studies of Europeans and 'natives' and could find no significant difference. This put in the dustbin a lot of protocols for 'acclimatizing' white men to the tropics. Actually, it probably didn't - you may be sure that many people persisted in doing the same-old same-old in the face of scientific evidence that they were wasting their time. More importantly he cracked the puzzle of the cause of beriberi, a progressive and often fatal neurological disease which killed native soldiers and prisoners in disproportionate numbers even if they were young and fit. Everyone had a theory, including 'vapours', rotten fish, and excessive perspiration. The Pekelharing and Winkler Commission followed the "it must have been something I ate" theory and tentatively identified a bacterial coccus as probable cause before they went home. Eijkman more of less accepted this, evidence based, theory until he noticed that the hospitals chickens developed a condition that looked suspiciously like beri-beri after they were fed on rice which was used to feed the native soldiery. They recovered when some martinet in the army refused to allow military rations to feed civilian birds! That was Eijkman's cue and after further research (convenient lab 'rat' now available) he was able to show that brown rice had a beneficial active principal that was lost when the rice was 'polished' to remove the husk.
Eijkman was successful in his endeavours at least partly because he paid attention to what was happening around him and he wasn't prepared to accept what other people considered certain - because they were all too often wrong. He is recognised by the Indonesian Government in the Eijkman Institute for Molecular Biology in downtown Jakarta. The Eijkman-Oxford Clinical Research Unit (EOCRU) was created in 2007 as a collaboration between the EIMB and Oxford University. There is also an Eijkman-Winkler Institute for Clinical Microbiology attached to Universiteit Utrecht where he was working when he won the Nobel Prize. He died the following year in November 1930. Laten we ons petje te verwijderen.