Well I'm delirah! My molecular biology class had to run some cut DNA down another agarose gel today. After our disastrous foray in the world of gels two weeks ago, I was apprehensive. The conCSIeit was that some DNA had been found at/on/in a crime scene and DNA had also been obtained from five suspects: the cook, the butler, the husband, the lover and the nephew-who-will-inherit-everything. Our team had to treat the DNA with a battery of restriction enzymes to break it up into unique small chunks that would 'uniquely' identify one of the suspects as The Perp. As always where I work, there's never quite enough equipment, so there were several bottlenecks in the process and things got delayed. The delay was made much worse by the fact that we (that is I - the buck stops with me) had loaded the gel-rig but not switched it on. So by the time everything should have been running, or indfeed finished, the class was over. Luckily I had a free hour immediately afterwards, so I was able to finish up for the class AND use another piece of kit to take a UV picture of the results. And the pictures were fabulous or, more accurately, 3 out of 4 of them could be interpreted in the required way. So I feel that, after an interval of some 25 years, I can finally add I can add "can run a gel-rig" to my CV.
Note for sleuths; the left hand track is the Crime Scene, the others are suspects A,B,C,D,E
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